PPP1R8 Polyclonal antibody

PPP1R8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16115-1-AP

Synonyms

NIPP1, NIPP 1, ARD-1, ARD1, ARD 1

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 16115-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16115-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16115-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: PC-3 cells, HepG2 cells
• Positive IHC detected in: human kidney tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16115-1-AP targets PPP1R8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R8 fusion protein Ag9041
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 8
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 47 kDa, 28 kDa, 19 kDa
• GenBank Accession Number: BC013360
• Gene Symbol: PPP1R8
• Gene ID (NCBI): 5511
• RRID: AB_2169304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.