PPP1R15B Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

PPP1R15B Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240052G5

Cat no : 83016-6-PBS

Synonyms

PPP1R15B;CReP, FLJ14744, CREP, 240052G5

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Validation Data Gallery

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  WB analysis using 83016-6-RR (same clone as 83016-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83016-6-RR (PPP1R15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 83016-6-RR (same clone as 83016-6-PBS)

  WB result of PPP1R15B antibody (83016-6-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83016-6-RR (same clone as 83016-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PPP1R15B antibody (83016-6-RR, Clone: 240052G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83016-6-RR (same clone as 83016-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug PPP1R15B Recombinant antibody (83016-6-RR, Clone:240052G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83016-6-PBS is PPP1R15B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PPP1R15B fusion protein Ag6213
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Calculated Molecular Weight: 79 kDa
• GenBank Accession Number: BC065280
• Gene Symbol: PPP1R15B
• Gene ID (NCBI): 84919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.