PPP1R15B Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP1R15B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (2)

Cat no : 14634-1-AP

Synonyms

FLJ14744, CREP

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Validation Data Gallery

• 

  WB analysis of SGC-7901 using 14634-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 14634-1-AP

  WB result of PPP1R15B antibody (14634-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells.

• 

  WB analysis of MCF-7 using 14634-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14634-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14634-1-AP

  IP result of anti-PPP1R15B (IP:14634-1-AP, 3ug; Detection:14634-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

• 

  IHC staining of mouse brain using 14634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14634-1-AP

  Immunofluorescent analysis of HepG2 cells using 14634-1-AP (PPP1R15B antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG .

• 

  IF Staining of SH-SY5Y using 14634-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP1R15B antibody (14634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A2780 cells, MCF-7 cells, HEK-293 cells, SGC-7901 cells, MDA-MB-231 cells, SKOV-3 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14634-1-AP targets PPP1R15B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R15B fusion protein Ag6213
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC065280
• Gene Symbol: PPP1R15B
• Gene ID (NCBI): 84919
• RRID: AB_2300036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Publications

Species	Application	Title
	WB	Nature; Stress response silencing by an E3 ligase mutated in neurodegeneration; Authors - Diane L Haakonsen
human	WB	Cell; Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.; Authors - Agnieszka Krzyzosiak
mouse	WB	Neuron; Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome; Authors - Mehdi Hooshmandi
mouse	WB	Hepatology; PMID: 29698569; Authors - Xu Xu ; KO Validated
human	WB,IF	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan
mouse	WB	Nat Commun; Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.; Authors - Hongtan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells; mouse, brain

PPP1R15B Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells; mouse, brain (Cat no:14634-1-AP)

FH

Will (Verified Customer) (10-24-2019)

good antibody, recommended by a colleague, would order again

• Applications: Western Blot,
• Cell Tissue Type: hepatocytes

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