PPP1R14B Polyclonal antibody

PPP1R14B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18476-1-AP

Synonyms

PHI 1, PLCB3N, PNG, PPP1R14B, SOM172

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Validation Data Gallery

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  WB analysis of MCF-7 using 18476-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18476-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18476-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 18476-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse liver tissue, human heart tissue, mouse skeletal muscle tissue, U2OS cells, A549 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

18476-1-AP targets PPP1R14B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R14B fusion protein Ag13373
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 14B
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC014522
• Gene Symbol: PPP1R14B
• Gene ID (NCBI): 26472
• RRID: AB_10598019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse		Immunity; Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis.; Authors - Kiyoharu Fukushima
human	WB,IHC	J Cell Mol Med; PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma; Authors - Kang He
human	WB	Biochim Biophys Acta Mol Basis Dis; Lysine lactylation-based insight to understanding the characterization of cervical cancer; Authors - Chaoran He