PPP1CA Monoclonal antibody, PBS Only

PPP1CA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C10A2

Cat no : 67070-1-PBS

Synonyms

PP 1A, PPP1A, PPP1CA

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Validation Data Gallery

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  WB analysis using 67070-1-Ig (same clone as 67070-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67070-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67070-1-Ig (same clone as 67070-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 67070-1-Ig (same clone as 67070-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 67070-1-Ig (same clone as 67070-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67070-1-Ig (same clone as 67070-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67070-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67070-1-PBS targets PPP1CA in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP1CA fusion protein Ag28451
• Full Name: protein phosphatase 1, catalytic subunit, alpha isoform
• Calculated Molecular Weight: 330 aa, 38 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001888
• Gene Symbol: PPP1CA
• Gene ID (NCBI): 5499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.