PPME1 Recombinant antibody

• 

  WB analysis using 85221-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85221-5-RR (PPME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PPME1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85221-5-RR

Clone No.242929H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, MCF-7 cells, K-562 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85221-5-RR targets PPME1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PPME1 fusion protein Ag5817
• Full Name: protein phosphatase methylesterase 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC050705
• Gene Symbol: PPME1
• Gene ID (NCBI): 51400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y570
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).