PPME1 Polyclonal antibody

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  WB analysis using 14435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 14435-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14435-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14435-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14435-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPME1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14435-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.1.89, PME 1, PME1, PME-1, PP2593

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, human brain tissue, fetal human brain tissue, A549 cells, rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14435-1-AP targets PPME1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPME1 fusion protein Ag5817
• Full Name: protein phosphatase methylesterase 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC050705
• Gene Symbol: PPME1
• Gene ID (NCBI): 51400
• RRID: AB_2284364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y570
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).

Publications

Species	Application	Title
human	WB	Front Immunol; CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses.; Authors - Xiaowen Bi
human	WB	Neural Regen Res; FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression; Authors - Jing Yao