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PDP1 Recombinant antibody, PBS Only (Detector)

PDP1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242090E7

Cat no : 84612-3-PBS

Synonyms

PPM2C, PDPC 1, PDP 1, EC:3.1.3.43, 242090E7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDP1 Recombinant antibody (84612-3-RR)

    WB analysis using 84612-3-RR (same clone as 84612-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84612-3-RR (PDP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01450-2

    Cytometric bead array standard curve of MP01450-2

    Cytometric bead array standard curve of MP01450-2, PPM2C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84612-1-PBS. Detection antibody: 84612-3-PBS. Standard: Ag15453. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 84612-3-PBS

    Affinity and Kinetic Characterization of 84612-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 84612-3-PBS against Human PDP1 was performed. The affinity constant is 358 nM.

  • Affinity and Kinetic Characterization of 84612-3-RR

    Affinity and Kinetic Characterization of 84612-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84612-3-RR against Human PDP1 were performed. The affinity constant is below 1 pM.

Product Information

84612-3-PBS targets PDP1 as part of a matched antibody pair:

MP01450-2: 84612-1-PBS capture and 84612-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDP1 fusion protein Ag15453
Full Name protein phosphatase 2C, magnesium-dependent, catalytic subunit
Calculated Molecular Weight 537 aa, 61 kDa
Observed Molecular Weight 55-61 kDa
GenBank Accession NumberBC047619
Gene Symbol PDP1
Gene ID (NCBI) 54704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Pyruvate dehydrogenase phosphatase 1 (PDP1),also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).