PPM1J Polyclonal antibody

PPM1J Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16269-1-AP

Synonyms

PPP2CZ, PP2C-zeta, PP2Czeta, PP2CZ, PP2C zeta

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Validation Data Gallery

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  WB analysis using 16269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16269-1-AP (PPM1J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16269-1-AP targets PPM1J in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1J fusion protein Ag9336
• Full Name: protein phosphatase 1J (PP2C domain containing)
• Calculated Molecular Weight: 505 aa, 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC011803
• Gene Symbol: PPM1J
• Gene ID (NCBI): 333926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1J also known as PPP2CZ, PP2C-zeta, belongs to the PP2C family. It has a unique N-terminal region and a proline-rich domain between conserved motifs II and III.