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PPM1G Polyclonal antibody

PPM1G Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15532-1-AP

Synonyms

PPM1C, PP2C-gamma, PP2CGAMMA, PP2CG, PP2C gamma



Tested Applications

Positive WB detected inHEK-293T cells, mouse lung tissue, Jurkat cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue, mouse testis tissue, human heart tissue, human kidney tissue, human skin tissue, human placenta tissue, human brain tissue, mouse spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15532-1-AP targets PPM1G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1G fusion protein Ag7751
Full Name protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC000057
Gene Symbol PPM1G
Gene ID (NCBI) 5496
RRIDAB_2169934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Protocols

Product Specific Protocols
WB protocol for PPM1G antibody 15532-1-APDownload protocol
IHC protocol for PPM1G antibody 15532-1-APDownload protocol
IF protocol for PPM1G antibody 15532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.

Authors - Mao-Xu Ge
humanWB

J Oncol

Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development

Authors - Weiran Liao
humanWB,IHC

Cell Death Dis

PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.

Authors - Dawei Chen
  • KD Validated