PPM1D Monoclonal antibody

PPM1D Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D6D5

Cat no : 67440-1-Ig

Synonyms

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, U2OS cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67440-1-Ig targets PPM1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1D fusion protein Ag24761
Full Name protein phosphatase 1D magnesium-dependent, delta isoform
Observed Molecular Weight 67 kDa, 47 kDa
GenBank Accession NumberBC060877
Gene Symbol PPM1D
Gene ID (NCBI) 8493
RRIDAB_2882676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.

Protocols

Product Specific Protocols
WB protocol for PPM1D antibody 67440-1-IgDownload protocol
IHC protocol for PPM1D antibody 67440-1-IgDownload protocol
IF protocol for PPM1D antibody 67440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols