PPM1B Monoclonal antibody, PBS Only

PPM1B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7H2

Cat no : 67647-1-PBS

Synonyms

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

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Validation Data Gallery

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  WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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Product Information

67647-1-PBS targets PPM1B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPM1B fusion protein Ag3871
• Full Name: protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
• Calculated Molecular Weight: 479 aa, 53 kDa
• Observed Molecular Weight: 36, 53 kDa
• GenBank Accession Number: BC012002
• Gene Symbol: PPM1B
• Gene ID (NCBI): 5495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.