PPM1B Polyclonal antibody

PPM1B Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

5

Cat no : 13193-1-AP

Synonyms

Protein phosphatase 1B, PP2C-beta, PP2CB, PP2C beta, EC:3.1.3.16

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Validation Data Gallery

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  WB analysis using 13193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13193-1-AP (PPM1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 13193-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13193-1-AP (PPM1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• IP: See 2 publications below

Product Information

13193-1-AP targets PPM1B in IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1B fusion protein Ag3871
• Full Name: protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
• Calculated Molecular Weight: 479 aa, 53 kDa
• Observed Molecular Weight: 36, 53 kDa
• GenBank Accession Number: BC012002
• Gene Symbol: PPM1B
• Gene ID (NCBI): 5495
• RRID: AB_10644118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1B is a member of the metal-dependent serine/threonine protein phosphatase (PPM) family with important regulatory functions in cellular signalling pathways(PMID: 18066953). PPM1B is also an important negative regulator of several stress signalling pathways and has been shown to negatively regulate the stress-activated p38 and JNK pathways by directly dephosphorylating upstream signalling proteins(PMID: 9824284). Moreover, PPM1B has also been shown to prevent cell death by negatively regulating necrotic cell death in a RIP3 dephosphorylation-dependent manner.(PMID: 25751141) PPM1B can be detected as the WM of 36-55 kDa.

Publications

Species	Application	Title
human	IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
mouse	IP,IF,Cell treatment	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane
human		Biochim Biophys Acta Mol Cell Res; TXLNA enhances TBK1 phosphorylation by suppressing PPM1B recruitment; Authors - Xiao Tian
mouse		Front Pharmacol; Initial Characterization of Stressed Transgenic Mice With Cardiomyocyte-Specific Overexpression of Protein Phosphatase 2C.; Authors - Paula Bollmann
human	IF	Exp Cell Res; RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities; Authors - Yueyang Li