PPIL2 Polyclonal antibody

PPIL2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12590-2-AP

Synonyms

CYC4, Cyclophilin 60, Cyp 60, CYP60, hCyP 60, PPIase, PPIL2, Rotamase PPIL2

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Validation Data Gallery

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  WB analysis of human kidney using 12590-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12590-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12590-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12590-2-AP

  IP result of anti-PPIL2 (IP:12590-2-AP, 4ug; Detection:12590-2-AP 1:300) with HEK-293 cells lysate 2600 ug.

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Tested Applications

• Positive WB detected in: human kidney tissue, HEK-293 cells, mouse kidney tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12590-2-AP targets PPIL2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPIL2 fusion protein Ag3296
• Full Name: peptidylprolyl isomerase (cyclophilin)-like 2
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC028385
• Gene Symbol: PPIL2
• Gene ID (NCBI): 23759
• RRID: AB_2169606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Front Cardiovasc Med; Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.; Authors - Yan Xu