PPIL1 Polyclonal antibody

PPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15144-1-AP

Synonyms

Peptidyl-prolyl cis-trans isomerase-like 1, hCyPX, EC:5.2.1.8, CYPL1, CGI-124

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 15144-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15144-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15144-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15144-1-AP

  IP result of anti-PPIL1 (IP:15144-1-AP, 3ug; Detection:15144-1-AP 1:400) with NIH/3T3 cells lysate 1600ug.

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  IHC staining of human colon cancer using 15144-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15144-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 15144-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 15144-1-AP (PPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A375 cells, HeLa cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below

Product Information

15144-1-AP targets PPIL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPIL1 fusion protein Ag7436
• Full Name: peptidylprolyl isomerase (cyclophilin)-like 1
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC003048
• Gene Symbol: PPIL1
• Gene ID (NCBI): 51645
• RRID: AB_2169603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	Neuron; Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly.; Authors - Guoliang Chai