PPIF-Specific Polyclonal antibody

PPIF-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 18466-1-AP

Synonyms

Cyclophilin F, Cyp D, CYP3, peptidylprolyl isomerase F, PPIase F, PPIF, Rotamase F

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Validation Data Gallery

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  WB analysis using 18466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 18466-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18466-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18466-1-AP

  IP result of anti-PPIF-Specific (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 18466-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human heart tissue, rat heart tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

18466-1-AP targets PPIF-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPIF-Specific fusion protein Ag13151
• Full Name: peptidylprolyl isomerase F
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC005020
• Gene Symbol: PPIF
• Gene ID (NCBI): 10105
• RRID: AB_2169273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF

Publications

Species	Application	Title
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
human	IHC	Aging (Albany NY); Overexpression of TICRR and PPIF confer poor prognosis in endometrial cancer identified by gene co-expression network analysis.; Authors - Linlin Yang
mouse	WB	J Biol Chem; The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.; Authors - Hakjoo Lee
human	IF	PLoS One; Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM.; Authors - Wang Yun E YE
mouse	WB	EMBO J; Prohibitin 1 regulates mtDNA release and downstream inflammatory responses; Authors - Hao Liu
human	WB	Biol Pharm Bull; Cinnamtannin B-1 inhibits the progression of osteosarcoma by regulating the miR-1281/PPIF axis; Authors - Jun Jia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Veronica (Verified Customer) (01-30-2024)

Really satisfied with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: 12% acrylammide gel Ab’: 1:2000 in 5% milk O/N

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

PPIF-Specific Antibody Western Blot validation (1:2000 dilution) in HeLa (Cat no:18466-1-AP)