CYPC Polyclonal antibody

• Featured Product
• KD/KO Validated

CYPC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10287-2-AP

Synonyms

PPIC, Rotamase C, PPIase C, Peptidyl-prolyl cis-trans isomerase C, EC:5.2.1.8

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Validation Data Gallery

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  WB analysis using 10287-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10287-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 10287-2-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10287-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 10287-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYPC antibody (10287-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 10287-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10287-2-AP (CYPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells, HEK-293 cells, mouse heart tissue
• Positive IHC detected in: human heart tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

10287-2-AP targets CYPC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYPC fusion protein Ag0209
• Full Name: peptidylprolyl isomerase C (cyclophilin C)
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC002678
• Gene Symbol: PPIC/CYPC
• Gene ID (NCBI): 5480
• RRID: AB_2169424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclophilin C also named as peptidyl-prolyl cis-trans isomerase C(PPIC), is expressed in several bone marrow (BM) hematopoietic progenitors and in T‐cell precursors(PMID: 33864384). CYPC/PPIC catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. In addition, CYPC/PPIC is involved in many biological processes, including mitochondrial metabolism, REDOX and inflammation, as well as related diseases and conditions, such as ischemia-reperfusion injury, AIDS and cancer.

Publications

Species	Application	Title
human	WB	J Biol Chem; The isomerase active site of cyclophilin A is critical for hepatitis C virus replication.; Authors - Udayan Chatterji ; KD Validated
human	WB	J Biol Chem; Depletion of Cyclophilins B and C Leads to Dysregulation of Endoplasmic Reticulum Redox Homeostasis.; Authors - Pawel Stocki
human	WB	PLoS One; Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules.; Authors - Daniel C Chapman
human	WB	Int J Mol Sci; Pharmacological Targeting of the ER-Resident Chaperones GRP94 or Cyclophilin B Induces Secretion of IL-22 Binding Protein Isoform-1 (IL-22BPi1).; Authors - Paloma Gómez-Fernández
human	WB	ACS Chem Neurosci; Furanoditerpenes from Spongia (Spongia) tubulifera Display Mitochondrial-Mediated Neuroprotective Effects by Targeting Cyclophilin D.; Authors - Rebeca Alvariño
mouse	WB	Front Physiol; Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells; Authors - Rebeca Alvariño