PPFIBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PPFIBP2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13953-1-AP

Synonyms

PTPRF-interacting protein-binding protein 2, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 2, Liprin-beta-2

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Validation Data Gallery

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  IHC staining of human stomach using 13953-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13953-1-AP (PPFIBP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 13953-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13953-1-AP (PPFIBP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 13953-1-AP

  Immunofluorescent analysis of A431 cells using 13953-1-AP (PPFIBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13953-1-AP targets PPFIBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPFIBP2 fusion protein Ag5064
• Full Name: PTPRF interacting protein, binding protein 2 (liprin beta 2)
• Calculated Molecular Weight: 99 kDa
• GenBank Accession Number: BC021714
• Gene Symbol: PPFIBP2
• Gene ID (NCBI): 8495
• RRID: AB_2877997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance; Authors - Mengting Xu
human	WB,IF	Biol Cell; Effects of the scaffold proteins liprin-α1, β1 and β2 on invasion by breast cancer cells.; Authors - Sara Chiaretti ; KD Validated