PPFIA3 Recombinant antibody

PPFIA3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230459F6

Cat no : 83142-4-RR

Synonyms

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654, 230459F6

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Validation Data Gallery

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  WB analysis using 83142-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83142-4-RR (PPFIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 83142-4-RR

  IP result of anti-PPFIA3 (IP:83142-4-RR, 4ug; Detection:83142-4-RR 1:4000) with mouse brain tissue lysate 2100 ug.

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  FC experiment of HEK-293 using 83142-4-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-251 using 83142-4-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83142-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83142-4-RR against Human PPFIA3 were performed. The affinity constant is 0.131 nM.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive FC (Intra) detected in: U-251 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83142-4-RR targets PPFIA3 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PPFIA3 fusion protein Ag1971
• Full Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
• Calculated Molecular Weight: 1194 aa, 133 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC021255
• Gene Symbol: PPFIA3
• Gene ID (NCBI): 8541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)