Liprin Alpha 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Liprin Alpha 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 14175-1-AP

Synonyms

PPFIA1, LIP-1, LIP1, LIP.1, LIP 1

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Validation Data Gallery

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  WB analysis using 14175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin Alpha 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14175-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14175-1-AP

  IP result of anti-Liprin Alpha 1 (IP:14175-1-AP, 4ug; Detection:14175-1-AP 1:500) with MCF-7 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14175-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Liprin Alpha 1 antibody (14175-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 20 publications below

Product Information

14175-1-AP targets Liprin Alpha 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Liprin Alpha 1 fusion protein Ag5381
• Full Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
• Calculated Molecular Weight: 1202 aa, 136 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC034046
• Gene Symbol: Liprin Alpha 1
• Gene ID (NCBI): 8500
• RRID: AB_2171592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Publications

Species	Application	Title
human	IF	Nat Commun; Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.; Authors - Xingqiao Xie
human	WB,IF	EMBO J; The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network; Authors - Cuirong Guan ; KD Validated
human	WB,IF	Nat Commun; PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.; Authors - Giulia Mana ; KD Validated
mouse	IF	Elife; Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion.; Authors - Dillon Jevon
human	WB	Mol Oncol; Liprin-α1 contributes to oncogenic MAPK signaling by counteracting ERK activity; Authors - Henna Pehkonen ; KD Validated
human	IF	Stem Cells Transl Med; Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix.; Authors - Reena Singh

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