PPAR Gamma Polyclonal antibody

• Featured Product
• KD/KO Validated

PPAR Gamma Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 22061-1-AP

Synonyms

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG2

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Validation Data Gallery

• 

  WB analysis using 22061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22061-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22061-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22061-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22061-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22061-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 22061-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 22061-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 22061-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22061-1-AP (PPAR gamma antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22061-1-AP (PPAR gamma antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, human heart tissue, mouse ovary tissue, mouse heart tissue, mouse testis tissue, mouse liver tissue, MCF-7 cells, 3T3-L1 cells, rat brain tissue, HL-60 cells, mouse brain tissue, rat liver tissue
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue, rat colon tissue, human placenta tissue ; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

22061-1-AP targets PPAR Gamma in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPAR Gamma fusion protein Ag17136
• Full Name: peroxisome proliferator-activated receptor gamma
• Calculated Molecular Weight: 505 aa, 58 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC006811
• Gene Symbol: PPARG
• Gene ID (NCBI): 5468
• RRID: AB_11182268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publications

Species	Application	Title
mouse	WB	Autophagy; Mir223 restrains autophagy and promotes CNS inflammation by targeting ATG16L1.; Authors - Yan Li
mouse	WB	Genes Dev; Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.; Authors - Stephanie Pearson
mouse	WB	Free Radic Biol Med; Hydrogen sulfide alleviates particulate matter-induced emphysema and airway inflammation by suppressing ferroptosis.; Authors - Ying Wang
mouse	WB	Biochem Pharmacol; A novel pyrazole-containing indolizine derivative suppresses NF-κB activation and protects against TNBS-induced colitis via a PPAR-γ-dependent pathway.; Authors - Yong Fu
		Front Pharmacol; The Active Components of Fuzheng Huayu Formula and Their Potential Mechanism of Action in Inhibiting the Hepatic Stellate Cells Viability - A Network Pharmacology and Transcriptomics Approach.; Authors - Xinrui Xing
rat	WB	Front Pharmacol; Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats.; Authors - Yuhang Ge

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