PPAPDC3 Polyclonal antibody

PPAPDC3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20635-1-AP

Synonyms

C9orf67, KIAA0515, NET39, PPAPDC3

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 20635-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20635-1-AP (PPAPDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20635-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20635-1-AP (PPAPDC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 20635-1-AP

  IP result of anti-PPAPDC3 (IP:20635-1-AP, 4ug; Detection:20635-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 20635-1-AP is PPAPDC3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPAPDC3 fusion protein Ag14676
• Full Name: phosphatidic acid phosphatase type 2 domain containing 3
• Calculated Molecular Weight: 271 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC006362
• Gene Symbol: PPAPDC3
• Gene ID (NCBI): 84814
• RRID: AB_10696180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPAPDC3(Phosphatidic acid phosphatase type 2 domain-containing protein 3) is also named as C9orf67 and belongs to the PA-phosphatase related phosphoesterase family. It plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling.

Publications

Species	Application	Title
human	WB	Genome Biol; Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.; Authors - Zuleger Nikolaj N
human	WB	Cells; Lamin A/C Assembly Defects in LMNA-Congenital Muscular Dystrophy Is Responsible for the Increased Severity of the Disease Compared with Emery-Dreifuss Muscular Dystrophy.; Authors - Anne T Bertrand
human	WB	Front Cell Dev Biol; Nuclear envelope transmembrane proteins involved in genome organization are misregulated in myotonic dystrophy type 1 muscle; Authors - Vanessa Todorow