PPAP2B Polyclonal antibody

PPAP2B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27391-1-AP

Synonyms

PAP2 beta, PAP2 b, PAP 2b, LPP3, EC:3.1.3.-

---

Validation Data Gallery

• 

  WB analysis using 27391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27391-1-AP (PPAP2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: U-87 MG cells, human placenta tissue, mouse cerebellum tissue, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27391-1-AP targets PPAP2B in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPAP2B fusion protein Ag26422
• Full Name: phosphatidic acid phosphatase type 2B
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC009196
• Gene Symbol: PPAP2B
• Gene ID (NCBI): 8613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidic acid phosphatase type 2B (PPAP2B), also known as Lipid phosphate phosphatase 3 (LPP3), is a member of the phosphatidic acid phosphatase (PAP) family. PPAP2B is an integral membrane protein that inactivates lysophosphatidic acid, was implicated in coronary artery disease (CAD) by genomewide association studies (PMID: 26034042). PPAP2B can be detected at least two immunoreactive bands with different mobility, ranging from 36 to 50 kDa, which may reflect oligomer formation and/or post-translational protein modifications, such as glycosylation (PMID: 28364255).