PPAN Polyclonal antibody, PBS Only

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  WB analysis using 11006-1-AP (same clone as 11006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11006-1-AP (PPAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11006-1-AP (same clone as 11006-1-PBS)

  WB result of PPAN antibody (11006-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAN transfected HEK-293 cells. This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 11006-1-AP (same clone as 11006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11006-1-AP (PPAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PPAN Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11006-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

11006-1-PBS targets PPAN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPAN fusion protein Ag1419
• Full Name: peter pan homolog (Drosophila)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC009833
• Gene Symbol: PPAN
• Gene ID (NCBI): 56342
• RRID: AB_2168040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQ55
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.