POU4F3 Polyclonal antibody
POU4F3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21509-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Neuro-2a cells, HL-60 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 6 publications below |
Product Information
The immunogen of 21509-1-AP is POU4F3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | POU4F3 fusion protein Ag16004 |
Full Name | POU class 4 homeobox 3 |
Calculated Molecular Weight | 338 aa, 37 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC104923 |
Gene Symbol | POU4F3 |
Gene ID (NCBI) | 5459 |
RRID | AB_2878872 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for POU4F3 antibody 21509-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Adv Sci (Weinh) Injectable, Antioxidative, and Tissue-Adhesive Nanocomposite Hydrogel as a Potential Treatment for Inner Retina Injuries | ||
Stem Cell Res Ther Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells | ||
Front Cell Dev Biol In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas. | ||
Sci Rep 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively. | ||
Neurochem Int Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH AMRITA (Verified Customer) (02-15-2021) | The antibody requires a prior antigen retrieval step for 15min in 80 deg centigrade
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