POU3F4 Polyclonal antibody
POU3F4 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 25114-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
The immunogen of 25114-1-AP is POU3F4 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | POU3F4 fusion protein Ag18925 |
Full Name | POU class 3 homeobox 4 |
Calculated Molecular Weight | 361 aa, 39 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC146551 |
Gene Symbol | POU3F4 |
Gene ID (NCBI) | 5456 |
RRID | AB_2879903 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OTF9 also named as BRN4 and POU3F4, belongs to the POU transcription factor family and Class-3 subfamily. OTF9 is a transcription factor which exerts its primary action widely during early neural development and in a very limited set of neurons in the mature brain. Mutation of OTF9 will cause of X-linked deafness type 3 (DFN3).
Protocols
Product Specific Protocols | |
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WB protocol for POU3F4 antibody 25114-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Eng An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors |