POU2AF1 Monoclonal antibody

POU2AF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C5A7

Cat no : 66659-1-Ig

Synonyms

BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1

---

Validation Data Gallery

• 

  WB analysis of Raji using 66659-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66659-1-Ig (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66659-1-Ig targets POU2AF1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: POU2AF1 fusion protein Ag23613
• Full Name: POU class 2 associating factor 1
• Calculated Molecular Weight: 256 aa, 27 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC032549
• Gene Symbol: POU2AF1
• Gene ID (NCBI): 5450
• RRID: AB_2882016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.