POP7 Polyclonal antibody

POP7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14964-1-AP

Synonyms

RPP20, RPP2, RNaseP protein p20, hPOP7

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Validation Data Gallery

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  WB analysis of HeLa using 14964-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14964-1-AP (POP7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14964-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14964-1-AP (POP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14964-1-AP

  IP result of anti-POP7 (IP:14964-1-AP, 4ug; Detection:14964-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 14964-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14964-1-AP (POP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14964-1-AP targets POP7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POP7 fusion protein Ag6827
• Full Name: processing of precursor 7, ribonuclease P/MRP subunit (S. cerevisiae)
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC001430
• Gene Symbol: POP7
• Gene ID (NCBI): 10248
• RRID: AB_2166488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.; Authors - Jian Zhang