POP5 Polyclonal antibody

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  WB analysis using 13146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13146-1-AP (POP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

POP5 Polyclonal Antibody for WB, ELISA

Cat No. 13146-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hPop5, HSPC004, POP5, RPP2, RPP20, x0003

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13146-1-AP targets POP5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POP5 fusion protein Ag3710
• Full Name: processing of precursor 5, ribonuclease P/MRP subunit (S. cerevisiae)
• Calculated Molecular Weight: 163 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC012505
• Gene Symbol: POP5
• Gene ID (NCBI): 51367
• RRID: AB_3669153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969H6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POP5 is a component of ribonuclease P and MRP ribonuclease complex that cleaves pre-rRNA sequences and generates mature tRNA molecules. Endogenous hPop5 protein is localized in the nucleus and accumulates in the nucleolus, which is consistent with its association with RNase MRP and RNase P. The human Pop5 cDNA encodes a protein of 163 amino acids with a predicted molecular mass of 18.8 kDa (PMID: 11413139).