POP1 Polyclonal antibody

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  WB analysis using 12029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12029-1-AP

  WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells.

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  IP experiment of HeLa using 12029-1-AP

  IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug.

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  IHC staining of human ovary cancer using 12029-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12029-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12029-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• RIP: See 2 publications below

Product Information

12029-1-AP targets POP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POP1 fusion protein Ag2660
• Full Name: processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
• Calculated Molecular Weight: 1024 aa, 115 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC011529
• Gene Symbol: POP1
• Gene ID (NCBI): 10940
• RRID: AB_2166477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99575
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.

Publications

Species	Application	Title
human	WB	Mol Cell; HSP70 binds to specific non-coding RNA and regulates human RNA polymerase III; Authors - Sergio Leone
human	WB	Mol Cell; Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.; Authors - Ok Hyun Park
human	WB	Nat Microbiol; The SARS-CoV-2 RNA-protein interactome in infected human cells.; Authors - Nora Schmidt
human	WB,RIP,IHC	Research (Wash D C); POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA; Authors - Chao Zhang
human	WB	Cell Rep; UPF1 promotes rapid degradation of m6A-containing RNAs.; Authors - Sung Ho Boo
human	IP,WB	Cell Rep; m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation; Authors - Sung Ho Boo ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Estefania (Verified Customer) (05-16-2022)

Many bands appearing. Protease inhibitor not used for the preparation of cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2 cell lysate

POP1 Antibody Western Blot validation (1:500 dilution) in HepG2 cell lysate (Cat no:12029-1-AP)