PON3 Recombinant antibody

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  WB analysis using 84604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84604-4-RR (PON3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84604-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84604-4-RR (PON3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84604-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84604-4-RR against Human PON3 were performed. The affinity constant is below 1 pM.

PON3 Recombinant Antibody for WB, IHC, ELISA

Cat No. 84604-4-RR

Clone No.242035D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1, 242035D11

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Tested Applications

• Positive WB detected in: A549 cells, HuH-7 cells, HepG2 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84604-4-RR targets PON3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PON3 fusion protein Ag36602
• Full Name: paraoxonase 3
• Calculated Molecular Weight: NM_000940.2 40kDa，354aa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: NM_000940.2
• Gene Symbol: PON3
• Gene ID (NCBI): 5446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q15166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.