PON2 Recombinant antibody

• 

  WB analysis using 85126-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85126-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PON2 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85126-4-RR

Clone No.242673H1

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: mouse heart tissue, L-929 cells, RAW 264.7 cells, mouse lung tissue, rat lung tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85126-4-RR targets PON2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: paraoxonase 2
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: NM_183308.2
• Gene Symbol: Pon2
• Gene ID (NCBI): 330260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62086
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).