PON2 Polyclonal antibody

PON2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14379-1-AP

Synonyms

Serum aryldialkylphosphatase 2, paraoxonase 2, EC:3.1.1.2, Aromatic esterase 2, A-esterase 2

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Validation Data Gallery

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  WB analysis of human liver using 14379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14379-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 14379-1-AP

  IP result of anti-PON2 (IP:14379-1-AP, 4ug; Detection:14379-1-AP 1:500) with L02 cells lysate 3200ug.

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14379-1-AP

  Immunofluorescent analysis of HepG2 cells, using PON2 antibody 14379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human liver tissue, L02 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

14379-1-AP targets PON2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PON2 fusion protein Ag5759
• Full Name: paraoxonase 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC046160
• Gene Symbol: PON2
• Gene ID (NCBI): 5445
• RRID: AB_2166330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).

Publications

Species	Application	Title
human	IHC	FASEB J; Breaking the chain at the membrane: paraoxonase 2 counteracts lipid peroxidation at the plasma membrane.; Authors - Henning Hagmann
mouse	WB	Am J Pathol; Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.; Authors - Joe Myung Kuk MK
human,mouse	IHC	Cells; Paraoxonase 2 (PON2) Deficiency Reproduces Lipid Alterations of Diabetic and Inflammatory Glomerular Disease and Affects TRPC6 Signaling; Authors - Henning Hagmann
mouse	WB,IF	Biomed Pharmacother; GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes; Authors - Xueqi Chen