POMP Recombinant antibody

POMP Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240563D7

Cat no : 83607-6-RR

Synonyms

PNAS 110, hUMP1, HSPC036, HSPC014, C13orf12

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Validation Data Gallery

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  WB analysis using 83607-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 83607-6-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83607-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83607-6-RR against Human POMP were performed. The affinity constant is 0.424 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, rat skin tissue, Jurkat cells, HepG2 cells, SW480 cells, HL-60 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83607-6-RR is POMP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: POMP fusion protein Ag6987
• Full Name: proteasome maturation protein
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC003390
• Gene Symbol: POMP
• Gene ID (NCBI): 51371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome maturation protein(POMP), is an essential factor of mammalian proteasome biogenesis. It facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. A single nucleotide deletion in this gene causes an autosomal recessive skin disorder, keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) syndrome.