POMC Monoclonal antibody, PBS Only

POMC Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3C12C7

Cat no : 66358-1-PBS

Synonyms

Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha, ACTH

---

Validation Data Gallery

• 

  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (66358-1-Ig, Clone: 3C12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human POMC (66358-1-Ig, Clone:3C12C7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

---

Product Information

66358-1-PBS targets POMC in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: POMC fusion protein Ag17311
• Full Name: proopiomelanocortin
• Calculated Molecular Weight: 267 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC065832
• Gene Symbol: POMC
• Gene ID (NCBI): 5443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.