POMC Monoclonal antibody, PBS Only

POMC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C12C7

Cat no : 66358-1-PBS

Synonyms

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin

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Validation Data Gallery

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  WB analysis of pig brain using 66358-1-Ig (same clone as 66358-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Transfected HEK-293 using 66358-1-Ig (same clone as 66358-1-PBS)

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66358-1-Ig (same clone as 66358-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 66358-1-Ig (same clone as 66358-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66358-1-Ig (same clone as 66358-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 66358-1-Ig (same clone as 66358-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 66358-1-Ig (same clone as 66358-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (66358-1-Ig, Clone: 3C12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66358-1-PBS targets POMC in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: POMC fusion protein Ag17311
• Full Name: proopiomelanocortin
• Calculated Molecular Weight: 267 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC065832
• Gene Symbol: POMC
• Gene ID (NCBI): 5443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.