POLR3H Polyclonal antibody

POLR3H Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16423-1-AP

Synonyms

RPC8, KIAA1665, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H

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Validation Data Gallery

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  WB analysis of HeLa using 16423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16423-1-AP (POLR3H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16423-1-AP (POLR3H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16423-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16423-1-AP (POLR3H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16423-1-AP targets POLR3H in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3H fusion protein Ag9624
• Full Name: polymerase (RNA) III (DNA directed) polypeptide H (22.9kD)
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC017248
• Gene Symbol: POLR3H
• Gene ID (NCBI): 171568
• RRID: AB_2878257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Subcellular localization shapes the fate of RNA polymerase III; Authors - Kai Tian