POLR3G Polyclonal antibody

POLR3G Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24701-1-AP

Synonyms

POLR3G, RNA polymerase III subunit C7, RPC32, RPC7

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Validation Data Gallery

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  WB analysis of HeLa using 24701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 24701-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24701-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24701-1-AP

  IP result of anti-POLR3G (IP:24701-1-AP, 3ug; Detection:24701-1-AP 1:500) with HeLa cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 24701-1-AP is POLR3G Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3G fusion protein Ag19872
• Full Name: polymerase (RNA) III (DNA directed) polypeptide G (32kD)
• Calculated Molecular Weight: 223 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC141649
• Gene Symbol: POLR3G
• Gene ID (NCBI): 10622
• RRID: AB_2879681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR3G also named as RNA polymerase III subunit C7 is a 223 amino acid protein, which belongs to eukaryotic RPC7 RNA polymerase subunit family. The molecular weight of POLR3G is 26 kDa. POLR3G localizes in the nucleus and is a component of the RNA polymerase III complex, which may be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.

Publications

Species	Application	Title
human	WB	Hum Genet; Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder; Authors - Aiko Iwata-Otsubo