POLR3B Polyclonal antibody

POLR3B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 16574-1-AP

Synonyms

C128, POLR3B, RNA polymerase III subunit C2, RPC2

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Validation Data Gallery

• 

  WB analysis of HepG2 using 16574-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16574-1-AP (POLR3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16574-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16574-1-AP (POLR3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16574-1-AP targets POLR3B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3B fusion protein Ag9856
• Full Name: polymerase (RNA) III (DNA directed) polypeptide B
• Calculated Molecular Weight: 1133 aa, 128 kDa
• Observed Molecular Weight: 128 kDa
• GenBank Accession Number: BC046238
• Gene Symbol: POLR3B
• Gene ID (NCBI): 55703
• RRID: AB_2168001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Cell Rep; Subcellular localization shapes the fate of RNA polymerase III; Authors - Kai Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Krishnendu (Verified Customer) (02-27-2020)

Didn't work at all

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T