POLR2H Polyclonal antibody

POLR2H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 15086-1-AP

Synonyms

RPABC3, hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide

---

Validation Data Gallery

• 

  WB analysis of HL-60 using 15086-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15086-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15086-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15086-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15086-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15086-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15086-1-AP

  IP result of anti-POLR2H (IP:15086-1-AP, 4ug; Detection:15086-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

• 

  IHC staining of human breast cancer using 15086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 15086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 15086-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15086-1-AP (POLR2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HL-60 cells, mouse kidney tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15086-1-AP targets POLR2H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR2H fusion protein Ag7166
• Full Name: polymerase (RNA) II (DNA directed) polypeptide H
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC000739
• Gene Symbol: POLR2H
• Gene ID (NCBI): 5437
• RRID: AB_2167826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

Publications

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	IHC	Oncol Lett; Identification of the key genes and pathways in prostate cancer.; Authors - Shutong Fan
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Yuan (Verified Customer) (12-13-2019)

Worked for human Hela cell. Nice staining at nucleoli area.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Hela cell