POLR1A Polyclonal antibody

POLR1A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20595-1-AP

Synonyms

A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

20595-1-AP targets POLR1A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR1A fusion protein Ag14543
Full Name polymerase (RNA) I polypeptide A, 194kDa
Calculated Molecular Weight 1720 aa, 195 kDa
Observed Molecular Weight 190-195 kDa
GenBank Accession NumberBC017115
Gene Symbol POLR1A
Gene ID (NCBI) 25885
RRIDAB_10949227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.

Protocols

Product Specific Protocols
WB protocol for POLR1A antibody 20595-1-APDownload protocol
IP protocol for POLR1A antibody 20595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell

RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination.

Authors - Sijie Liu
humanIP

Cancer Sci

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.

Authors - Chaoshen Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5micrograms)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
POLR1A Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:20595-1-AP)