POLQ Polyclonal antibody

POLQ Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28590-1-AP

Synonyms

DKFZp781A0112, DNA polymerase theta variant, Homo sapiens (Human), POLH, POLQ, PRO0327

---

Validation Data Gallery

• 

  WB analysis using 28590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 28590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 28590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HuH-7 cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: A375 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28590-1-AP targets POLQ in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLQ fusion protein Ag29643
• Full Name: polymerase (DNA directed), theta
• Calculated Molecular Weight: 290 kDa
• Observed Molecular Weight: 250~290 kDa, 70~110 kDa
• GenBank Accession Number: NM_199420
• Gene Symbol: POLQ
• Gene ID (NCBI): 10721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).