POLG Recombinant antibody

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  WB analysis using 83670-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 83670-1-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 83670-1-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83670-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83670-1-RR against Human POLG were performed. The affinity constant is below 1 pM.

POLG Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83670-1-RR

Clone No.240441D2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase, 3'-5' exodeoxyribonuclease

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Tested Applications

• Positive WB detected in: HeLa cells, rat brain tissue, rat testis tissue, HepG2 cells, HEK-293T cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83670-1-RR targets POLG in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: POLG fusion protein Ag5256
• Full Name: polymerase (DNA directed), gamma
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC042571
• Gene Symbol: POLG
• Gene ID (NCBI): 5428
• RRID: AB_3671275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P54098
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLG (DNA polymerase gamma) is composed of a C-terminal polymerase ('pol') domain and an amino-terminal exonuclease ('exo') domain. The exo domain increases the fidelity of mitochondrial DNA replication by conferring a proofreading activity to the enzyme (PMID: 12210792). Catalytic subunit of POLG solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).