POLG Polyclonal antibody

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  WB analysis using 14110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14110-1-AP (POLG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

POLG Polyclonal Antibody for WB, ELISA

Cat No. 14110-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:4.2.99.-, EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14110-1-AP targets POLG in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLG fusion protein Ag5256
• Full Name: polymerase (DNA directed), gamma
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC042571
• Gene Symbol: POLG
• Gene ID (NCBI): 5428
• RRID: AB_3669178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54098
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLG is the catalytic subunit of DNA polymerase gamma solely responsible for the replication of mitochondrial DNA (mtDNA). POLG replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers, and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).