• Featured Product
  • KD/KO Validated

POLD3 Polyclonal antibody

POLD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21935-1-AP

Synonyms

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3



Tested Applications

Positive WB detected inMCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21935-1-AP is POLD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLD3 fusion protein Ag16570
Full Name polymerase (DNA-directed), delta 3, accessory subunit
Calculated Molecular Weight 466 aa, 51 kDa
Observed Molecular Weight 51-70 kDa
GenBank Accession NumberBC108908
Gene Symbol POLD3
Gene ID (NCBI) 10714
RRIDAB_2878949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLD3 antibody 21935-1-APDownload protocol
IHC protocol for POLD3 antibody 21935-1-APDownload protocol
IP protocol for POLD3 antibody 21935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dev

Break-induced replication promotes fragile telomere formation.

Authors - Zhe Yang
  • KO Validated
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
  • KD Validated
humanWB

J Adv Res

Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA

Authors - Xi Dong
human

Mol Cell

Mechanisms of insertions at a DNA double-strand break

Authors - Jaewon Min
mouseWB

Nucleic Acids Res

Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model

Authors - Nada Kojak
mouseWB

Nucleic Acids Res

Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres

Authors - Jennifer J Lee