POLD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

POLD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21935-1-AP

Synonyms

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3

---

Validation Data Gallery

• 

  WB analysis using 21935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21935-1-AP (POLD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 21935-1-AP

  IP result of anti-POLD3 (IP:21935-1-AP, 4ug; Detection:21935-1-AP 1:500) with MCF-7 cells lysate 640 ug.

• 

  IHC staining of human breast cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below

Product Information

The immunogen of 21935-1-AP is POLD3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLD3 fusion protein Ag16570
• Full Name: polymerase (DNA-directed), delta 3, accessory subunit
• Calculated Molecular Weight: 466 aa, 51 kDa
• Observed Molecular Weight: 51-70 kDa
• GenBank Accession Number: BC108908
• Gene Symbol: POLD3
• Gene ID (NCBI): 10714
• RRID: AB_2878949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Genes Dev; Break-induced replication promotes fragile telomere formation.; Authors - Zhe Yang ; KO Validated
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang ; KD Validated
human	WB	J Adv Res; Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA; Authors - Xi Dong
human		Mol Cell; Mechanisms of insertions at a DNA double-strand break; Authors - Jaewon Min
mouse	WB	Nucleic Acids Res; Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model; Authors - Nada Kojak
mouse	WB	Nucleic Acids Res; Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres; Authors - Jennifer J Lee