POLD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

POLD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 15646-1-AP

Synonyms

CDC2, POLD, POLD1

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Validation Data Gallery

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  WB analysis using 15646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15646-1-AP

  IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 15646-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15646-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15646-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15646-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 2 publications below

Product Information

15646-1-AP targets POLD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLD1 fusion protein Ag8178
• Full Name: polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• Calculated Molecular Weight: 1107 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC008800
• Gene Symbol: POLD1
• Gene ID (NCBI): 5424
• RRID: AB_2252572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )

Publications

Species	Application	Title
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling
human	WB	Nat Microbiol; Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation.; Authors - Lei Wei
human	WB	Nat Commun; Hepatitis B virus cccDNA is formed through distinct repair processes of each strand.; Authors - Lei Wei
human	WB	Hum Mol Genet; Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.; Authors - Ki Soon Kim
human	WB	Hum Mol Genet; ISCA1 mutation in a patient with infantile-onset leukodystrophy causes defects in mitochondrial [4Fe-4S] proteins.; Authors - Alessandra Torraco