PNPT1 Monoclonal antibody

PNPT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F2A1

Cat no : 68309-1-Ig

Synonyms

3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1

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Validation Data Gallery

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  WB analysis using 68309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IF Staining of HeLa using 68309-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, LNCap cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68309-1-Ig targets PNPT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PNPT1 fusion protein Ag6290
• Full Name: polyribonucleotide nucleotidyltransferase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC053660
• Gene Symbol: PNPT1
• Gene ID (NCBI): 87178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.