Pancreatic Lipase Polyclonal antibody

Pancreatic Lipase Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11209-1-AP

Synonyms

Lipase, Pancreatic Lipase, PL, PNLIP, PTL

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Validation Data Gallery

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  WB analysis of BxPC-3 using 11209-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11209-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11209-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, human pancreas tissue, mouse pancreas tissue
• Positive IHC detected in: mouse pancreas tissue, human pancreas cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

11209-1-AP targets Pancreatic Lipase in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pancreatic Lipase fusion protein Ag1681
• Full Name: pancreatic lipase
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC014309
• Gene Symbol: PNLIP
• Gene ID (NCBI): 5406
• RRID: AB_2167443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The pancreatic triacylglycerol lipase precursor (PNLIP), a 56 kDa protein secreted by the pancreas that is essential for the hydrolysis and absorption of long-chain triglyceride fatty acids in the intestine is located ~1 Mb upstream from the chromosome 10 peak(PMID:17890784). It is also named as PL, PTL and belongs to the AB hydrolase superfamily. It may potentially be involved in the pathophysiology of traumatic brain injury and may be implicated in the proliferation of astrocytes and the recovery of neurological outcomes(PMID:19760487). The full length protein has a signal peptide with 16 amino acid and a glycosylation site.

Publications

Species	Application	Title
mouse	IHC	Int J Mol Sci; Blocking β₁/β₂-Adrenergic Signaling Reduces Dietary Fat Absorption by Suppressing Expression of Pancreatic Lipase in High Fat-Fed Mice.; Authors - Kyunghwa Baek
pig	WB	JPEN J Parenter Enteral Nutr; Early Enteral Administration of a Complex Lipid Emulsion Supplement Prevents Postnatal Deficits in Docosahexaenoic and Arachidonic Acids and Increases Tissue Accretion of Lipophilic Nutrients in Preterm Piglets.; Authors - Olajumoke Akinsulire
human	IHC	Am J Cancer Res; A human pan-cancer system analysis of heat shock protein family A member 5; Authors - Chao Deng