PMS2 Polyclonal antibody

PMS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18164-1-AP

Synonyms

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

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Validation Data Gallery

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  WB analysis using 18164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18164-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18164-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 18164-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, PC-3 cells, Jurkat cells, MCF-7 cells, Raji cells
• Positive IHC detected in: human ovary cancer tissue, human colon cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18164-1-AP targets PMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMS2 fusion protein Ag12651
• Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Calculated Molecular Weight: 862 aa, 96 kDa
• Observed Molecular Weight: 117 kDa, 100 kDa
• GenBank Accession Number: BC093921
• Gene Symbol: PMS2
• Gene ID (NCBI): 5395
• RRID: AB_2167145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). This antibody is specific to PMS2. The calculated molecular weight of PMS2 is 96 kDa, but the modified PMS2 is about 117 kDa.(PMID: 11358834)