• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PMS1 Monoclonal antibody, PBS Only

PMS1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A6G3

Cat no : 68413-1-PBS

Synonyms

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PMS1 Monoclonal antibody (68413-1-Ig)

    WB analysis using 68413-1-Ig (same clone as 68413-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68413-1-Ig (PMS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68413-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PMS1 Monoclonal antibody (68413-1-Ig)

    IF Staining of Caco-2 using 68413-1-Ig (same clone as 68413-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PMS1 antibody (68413-1-Ig, Clone: 1A6G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68413-1-PBS in a different storage buffer formulation.

Product Information

68413-1-PBS targets PMS1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PMS1 fusion protein Ag28127
Full Name PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
Observed Molecular Weight 106 kDa
GenBank Accession NumberBC096331
Gene Symbol PMS1
Gene ID (NCBI) 5378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.