Tested Applications
| Positive WB detected in | JAR cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32470-1-AP targets PLXND1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLXND1 fusion protein Ag38374 Predict reactive species |
| Full Name | plexin D1 |
| Calculated Molecular Weight | 212kDa，1925aa |
| Observed Molecular Weight | 220-250 |
| GenBank Accession Number | NM_015103.2 |
| Gene Symbol | PLXND1 |
| Gene ID (NCBI) | 23129 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y4D7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLXND1, also known as KIAA0620. It is predicted to be located in the cell membrane. Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. It is a cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E, which plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. The protein regulates endothelial cell migration. The molecular weight of PLXND1 is 212 kDa, and the target also has phosphorylation modification as well.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLXND1 antibody 32470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
